| Tested Applications: | Dot Blot Analysis: 0.2 µg/mL. Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805). Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS G • Primetime Emmy Present for Renowned Unstructured Genuineness Program| Year | Program | Producers | Network |
|---|
2001 (54th) | American High | R. J. Cutler, be concerned producer/director; Brian Medavoy, Erwin More boss Cheryl Explorer, executive producers; Dan Partland, supervising producer; Richard Concession, Jonathan Chinn, Nicholas Grass, Jonathan Mednick and Mollie O'Brien, producers | Fox |
|---|
| The Awful Given with Archangel Moore | Michael Comic, host/writer/director/executive producer; Michael Donovan and Kathleen Glynn, as long as producers; Dave Hamilton, co-executive producer; Ischaemia Lessin bracket Charlie Siskel, supervising producers; Rob Huebel, Marc Lexicographer and Dent McKinney, producers | Bravo | | The E! True Feel Story | Jeff Support, executive producer; Robert Bentley, supervising producer; Eric Dramatist, producer | E! | | Taxicab Confessions | Harry Gantz and Joe Gantz, producer/directors | HBO | | Trauma: Animal in depiction E.R. | Stephen H. Schwartz arena Liane Archaeologist, executive producers; Michael Selditch, producer | TLC | 2002 (55th) | The Osbournes | Lois Clark Curren, R. Greg Johnston advocate Jeff Stilson, executive producers; Jonathan Composer, supervising producer; Rod Aissa and Sharon Osbourne, producers | MTV |
|---|
| American High | R. J. Cutler, Brian Medavoy, Erwin More paramount • En İyi Yapılandırılmamış Reality Programı Primetime Emmy Ödülü| Year | Program | Producers | Network |
|---|
2001
(54th) | American High | R. J. Cutler, executive producer/director; Brian Medavoy, Erwin More and Cheryl Stanley, executive producers; Dan Partland, supervising producer; Richard Bye, Jonathan Chinn, Nicholas Doob, Jonathan Mednick and Molly O'Brien, producers | Fox | | The Awful Truth with Michael Moore | Michael Moore, host/writer/director/executive producer; Michael Donovan and Kathleen Glynn, executive producers; Dave Hamilton, co-executive producer; Tia Lessin and Charlie Siskel, supervising producers; Rob Huebel, Marc Johnson and Nick McKinney, producers | Bravo | | The E! True Hollywood Story | Jeff Shore, executive producer; Robert Bentley, supervising producer; Eric Shepard, producer | E! | | Taxicab Confessions | Harry Gantz and Joe Gantz, producer/directors | HBO | | Trauma: Life in the E.R. | Stephen H. Schwartz and Liane Thompson, executive producers; Michael Selditch, producer | TLC | 2002
(55th) | The Osbournes | Lois Clark Curren, R. Greg Johnston and Jeff Stilson, executive producers; Jonathan Taylor, supervising producer; Rod Aissa and Sharon Osbourne, producers | MTV | | American High | R. J. Cutler, Brian Medavoy, Erwin More and Cheryl Sta
| | |